Imagej overlay images 64 Bit#

To merge several images, go to Image > Color > Merge Channels.To pseudocolor the image, go to Image > LookUp Table and select desired color.To change your image, go to Image > Type and select desired type.Ĭolor Processing: Make sure the selected channel or image is the primary window. Since this creates an overlay of the scale bar, you need to combine your image and the overlay by going to Image > Overlay > Flatten.Ĭhanging image type: Some microscopes create a higher quality 16-bit image, but 8-bit images work best to allow you to view them on your computer without any analysis software. To open a Z-stacked image, ensure you are splitting channels if a multi-channeled Z-stack.Īdding a scale bar: Go to Analyze > Tools > Scalebar.Ensure that the channels are colored correctly, as Fiji can occasionally misread the metadata. You can re-merge the channels later if desired. Splitting channels allows you to alter the channel color, if necessary. To open a multi-channel image, open your image the same way as a single channel and choose to “Split Channels”.Ensure the image is opened as a Hyperstack.If the image is a life science image file, the bio-formats window will open. To open a single channel image, go to File > Open and select your desired image.To ensure your image will open, use Fiji rather than standard ImageJ. czi files (or any other proprietary life science files) without the Bio-Formats plugin. Look under device specifications and system type.

Imagej overlay images 64 Bit#

If you are unsure if you have a 64 bit or a 32 bit OS, type “See if you have a 32-bit or 64-bit OS” into your computer’s search bar or go to Computer Settings > About.

Determine the appropriate download for your operating system. To download Fiji, go to and scroll until you see the download section. For further instruction, please refer to the NIH’s ImageJ user guide and other Fiji documentation. This brief guide will outline several basic processing and analysis methods.